[Molecular imprinting-flow injection chemiluminescence method for determination of doxycycline hydrochloride]

Doxycycline hydrochloride can enhance the chemiluminescence of potassium ferricyanide and luminol in alkaline medium. So a molecular imprinting-flow-injection chemiluminescence method for the determination of doxycycline hydrochloride was established by using doxycycline hydrochloride-imprinted polymers as recognition material and potassium ferricyanide and luminol as detection system. Doxycycline hydrochloride-imprinted polymer was synthesized using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. The linear range is 9.0 x 10(-7)-6.0 x 10(-5) g x mL(-1) and the detection limit is 3.2 x 10(-7) g x mL(-1). The relative standard deviation for 6.0 X 10(-6) g x mL(-1) of doxycycline hydrochloride was 3.5% (n = 9). This method has been successfully applied to the determination of doxycycline hydrochloride in tablets and in urine samples.