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Related Experiment Video

Updated: Jun 19, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Submicrometer tomographic resolution examined using a micro-fabricated test object.

Ryuta Mizutani¹, Akihisa Takeuchi, R Yoshiyuki Osamura

¹Department of Applied Biochemistry, School of Engineering, Tokai University, Kitakaname 1117, Hiratsuka, Kanagawa 259-1292, Japan. ryuta@tokai-u.jp

Micron (Oxford, England : 1993)

|October 6, 2009

Summary

This summary is machine-generated.

This study determined the spatial resolution of microtomography for visualizing submicrometer tissue structures. Zoom reconstruction achieved 0.8 micrometer in-plane resolution, comparable to through-plane resolution, enabling subcellular visualization.

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Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

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Published on: April 13, 2016

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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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Published on: August 16, 2012

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Area of Science:

Materials Science and Engineering
Biomedical Imaging and Instrumentation
Cell Biology

Background:

Human tissues possess micrometer and submicrometer structures crucial for biological function.
Accurate three-dimensional (3D) visualization of these microstructures is essential for understanding tissue biology and pathology.
Microtomography is a key technique for 3D imaging, but its spatial resolution needs precise evaluation.

Purpose of the Study:

To quantitatively estimate the spatial resolution of microtomography systems.
To assess the feasibility of visualizing submicrometer structures in human tissues using microtomography.
To compare in-plane and through-plane resolutions and identify optimal imaging parameters.

Main Methods:

Fabrication of a submicrometer test object using focused ion beam milling.
Microtomographic analysis of the test object to determine spatial resolution.
Calculation of in-plane and through-plane resolutions using modulation transfer function (MTF) analysis of square-wave patterns.

Main Results:

Non-zoom microtomography yielded an in-plane resolution of 1.2 micrometers.
Zoom reconstruction significantly improved in-plane resolution to 0.8 micrometers, matching the through-plane resolution.
Submicrometer 3D analysis successfully visualized subcellular structures in human cerebral tissue.

Conclusions:

Microtomography, particularly with zoom reconstruction, can achieve submicrometer spatial resolution.
This resolution is sufficient for visualizing intricate subcellular structures in human tissues.
The technique holds promise for detailed structural studies of biological samples.