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Updated: Sep 12, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
[Influence of phospholipids of different natures on the structure of ferricytochrome c]
Abstract:
Influence of the two types of lipid molecules (neutral and acidic phospholipids) on the structure of ferricytochrome c in lipid-protein complexes was studied with the use of circular dichroism and absorption spectroscopy methods. It was found that interaction of ferricytochrome c with acidic phospholipids (cardiolipin and phosphatidylinositols) brings about some changes in the protein conformation, while interaction with neutral phospholipid (phosphatidylcholine plus 10% lauric acid) does not. Some difference in the mode of interaction of different acidic phospholipids with ferricytochrome c was also observed. As evidenced by visual light absorption spectra, cardiolipin induced the disruption of hem--methionine 80 bond in the protein molecule, while phosphatidylinositols do not cause such an effect.
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