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Updated: Jun 19, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High-throughput approaches for miRNA expression analysis.

Cheng Lu1, Frédéric Souret

  • 1DuPont Agricultural Biotechnology, RT 141 & Henry Clay, Wilmington, DE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 6, 2009
PubMed
Summary
This summary is machine-generated.

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MicroRNAs (miRNAs) regulate gene expression but analyzing individual miRNA genes is challenging due to multiple related family members. This chapter details methods for miRNA expression analysis, aiding functional studies.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small RNA molecules crucial for regulating gene expression in plants and animals.
  • miRNA expression patterns are vital for understanding their functions, but challenges arise from multiple paralogous loci within miRNA families.
  • Distinguishing between closely related miRNA family members that produce identical mature miRNAs complicates individual gene analysis.

Purpose of the Study:

  • To describe common methods for analyzing microRNA (miRNA) expression.
  • To address the challenges in analyzing individual miRNA genes within complex families.
  • To provide insights into understanding miRNA functions through expression profiling.

Main Methods:

  • High-throughput sequencing for comprehensive miRNA expression profiling.

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Related Experiment Videos

Last Updated: Jun 19, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

  • Microarray analysis for large-scale miRNA expression quantification.
  • Brief discussion of quantitative real-time PCR (qRT-PCR) and Northern blotting for data validation.

    • Main Results:

    • The chapter outlines established techniques for quantifying miRNA levels.
    • It highlights the utility of sequencing and microarrays for studying miRNA expression.
    • Validation methods like qRT-PCR and Northern blotting are mentioned for confirming expression data.

    Conclusions:

    • Accurate miRNA expression analysis is essential for deciphering their regulatory roles.
    • Advanced techniques like high-throughput sequencing and microarrays facilitate robust expression profiling.
    • These methods help overcome challenges posed by miRNA gene families, enabling better functional interpretation.