Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Brain Imaging01:14

Brain Imaging

Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic Stimulation (TMS).
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Target ALS Global Natural History Study: Cross-platform proteomics to accelerate biofluid biomarker and drug target discovery in amyotrophic lateral sclerosis.

medRxiv : the preprint server for health sciences·2026
Same author

A standardized framework resolves ambiguity in motor neuron loss across neurodegenerative diseases.

bioRxiv : the preprint server for biology·2026
Same author

A mouse model of CHCHD10 p.R15L familial ALS presents mild, age-related motor neuron degeneration without protein instability or mitochondrial dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

SpIC3D imaging for spinal in situ contrast 3D visualization.

Cell reports methods·2025
Same author

Cerebellar pathology contributes to neurodevelopmental deficits in spinal muscular atrophy.

Brain : a journal of neurology·2025
Same author

Synaptic imbalance and increased inhibition impair motor function in SMA.

Science advances·2025

Related Experiment Video

Updated: Jun 19, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
08:26

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

Imaging nervous system activity.

Douglas R Fields1, Neil Shneider, George Z Mentis

  • 1Section on Nervous System Development and Plasticity, National Institute of Child Health and Human Development (NICHD), NIH, Bethesda, Maryland, USA.

Current Protocols in Neuroscience
|October 6, 2009
PubMed
Summary
This summary is machine-generated.

This study details methods for loading ion- and voltage-sensitive dyes into neurons to visualize neural activity. These techniques, initially focused on the spinal cord, are broadly applicable across the nervous system.

More Related Videos

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
07:04

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Published on: January 7, 2019

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution
06:18

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jun 19, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
08:26

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
07:04

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Published on: January 7, 2019

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution
06:18

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution

Published on: November 21, 2023

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Neurons utilize ion and voltage changes for signaling.
  • Visualizing neural activity is crucial for understanding nervous system function.
  • Specific dyes can report on these electrical changes.

Purpose of the Study:

  • To describe protocols for loading ion- and voltage-sensitive dyes into neurons.
  • To demonstrate the visualization of neural activity using these dyes.
  • To highlight the adaptability of these methods for various neural systems.

Main Methods:

  • Loading neurons with ion-sensitive dyes.
  • Loading neurons with voltage-sensitive dyes.
  • Utilizing fluorescence microscopy to detect dye signals.

Main Results:

  • Successful loading of dyes into spinal cord neurons.
  • Visualization of neural network activity in vitro.
  • Demonstration of dye utility in cultured and intact preparations.

Conclusions:

  • Established protocols enable effective dye loading for neural activity visualization.
  • Spinal cord preparations serve as a valuable model system.
  • Methods are transferable to diverse regions of the nervous system.