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Related Experiment Video

Updated: Jun 19, 2026

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Bioinformatics construction of the human cell surfaceome.

J P C da Cunha¹, P A F Galante, J E de Souza

¹São Paulo Branch, Ludwig Institute for Cancer Research, 01323-903 São Paulo, Brazil.

Proceedings of the National Academy of Sciences of the United States of America

|October 7, 2009

Summary

Researchers cataloged 3,702 human cell surface proteins (cell surfaceome) to identify potential diagnostic and therapeutic targets for cancer. This resource aids in discovering new cell surface cancer targets.

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Area of Science:

Genomics
Proteomics
Bioinformatics

Background:

Cell surface proteins are crucial targets for medical interventions.
A comprehensive catalog of these proteins is needed for cancer research.

Purpose of the Study:

To create a catalog of the human cell surfaceome.
To identify cell surface proteins with potential as diagnostic and therapeutic targets, particularly for tumors.
To analyze the genetic diversity and expression patterns of the cell surfaceome.

Main Methods:

Bioinformatics tools were used to generate the human cell surfaceome catalog.
Gene expression data from various sources were integrated.
Quantitative real-time PCR was employed for validation of selected surfaceome genes.

Main Results:

A catalog of 3,702 human cell surface proteins was generated.
Genes with restricted normal tissue expression and/or differential tumor expression were identified.
Potential diagnostic and therapeutic targets for colorectal tumors and glioblastoma were discovered.
Several cell surface cancer/testis antigens were identified.

Conclusions:

The human cell surfaceome catalog provides a valuable reference for identifying cell surface targets.
This resource facilitates the discovery of novel biomarkers and therapeutic strategies for various cancers.