Related Experiment Video
Updated: Jun 19, 2026

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
Published on: April 27, 2021
Unravelling Escherichia coli dynamics close to the maximum growth temperature through heterogeneous modelling
E Van Derlinden1, K Bernaerts, J F Van Impe
1Department of Chemical Engineering, Katholieke Universiteit Leuven, Belgium.
Aims:
Previous work showed that the exponential phase of Escherichia coli K12 MG1655, grown in Brain Heart Infusion broth at temperatures close to its maximum growth temperature, is disturbed. Based on plate count data, microscopic images and literature, the existence of a heat-resistant subpopulation was hypothesized. Here, this hypothesis is mathematically explored via a heterogeneous model.
Methods And Results:
A heat-sensitive and a heat-resistant subpopulation are considered. A large fraction of the population is inactivated, while the remaining smaller fraction is able to resist (or adapt to) the inimical temperature and grows. A heterogeneous model that encloses a growth model (resistant population) and an inactivation model (sensitive population) is used to describe the global population dynamics. Most experimental data can be predicted when taking parameter uncertainty via Monte Carlo simulation into account.
Conclusions:
The heterogeneous model accurately describes disturbed growth curves at superoptimal temperatures, except for high initial cell densities.
Significance And Impact Of The Study:
This study strengthens the hypothesis of the existence of a (small) heat-resistant subpopulation in typical inoculum cultures of E. coli K12 MG1655.
Related Concept Videos
Modeling with Differential Equations
Exponential Equations for Modeling Growth
Bacterial Growth Curve
Stringent Response in E. coli
Evolution of New Traits in Microbes
Factors Influencing Microbial Growth: Temperature

