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Characterization of Bacillus polyfermenticus KJS-2 as a probiotic
Kang Min Kim1, Myo Jeong Kim, Dong Hee Kim
1Department of Smart Foods and Drugs, Inje University, Gyeongsangnam-do 621-749, Korea.
Journal of Microbiology and Biotechnology
|October 8, 2009
Summary
Bacillus polyfermenticus KJS-2 efficiently converts glucose to lactic acid. This probiotic bacterium demonstrates high viability in simulated gastric conditions and strong adhesion to intestinal cells.
Area of Science:
- Microbiology
- Probiotics
- Bacterial Physiology
Background:
- Bacillus species are known for their potential probiotic properties.
- Characterization of novel strains is crucial for identifying beneficial microorganisms.
Purpose of the Study:
- To identify and characterize Bacillus polyfermenticus KJS-2.
- To evaluate its potential as a probiotic agent.
Main Methods:
- Transmission Electron Microscopy (TEM) for morphology.
- API 50CHB kit for biochemical profiling.
- 16S rDNA sequencing and phylogenetic analysis for identification.
- Catalase and oxidase tests for enzymatic activity.
- In vitro assays for glucose to lactic acid conversion, acid/bile tolerance, and Caco-2 cell adhesion.
Main Results:
- Bacillus polyfermenticus KJS-2 was successfully identified and characterized.
- Achieved a glucose to lactic acid conversion rate of 60.71+/-4.9%.
- Demonstrated high viability (140+/-7.9%) in artificial gastric juice (pH 2.0) and bile acid (108+/-3.5%, pH 6.5) after 4 hours.
- Exhibited significant adherence to Caco-2 cells, approximately 65+/-0.6%.
Conclusions:
- Bacillus polyfermenticus KJS-2 possesses robust probiotic potential due to its metabolic capabilities and resilience.
- Its ability to withstand harsh gastrointestinal conditions and adhere to intestinal cells supports its use in functional foods or supplements.
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