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Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements
Published on: July 13, 2016
Effects of ammonium on the antioxidative response in Hydrilla verticillata (L.f.) Royle plants
Chao Wang1, Song He Zhang, Pei Fang Wang
1Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environmental Science and Engineering, Hohai University, Nanjing 210098, China.
Abstract:
To investigate ammonium toxicity, the submerged plant Hydrilla verticillata (L.f.) Royle was treated with 0.1-3.0mM ammonium for 12h and 4d. After exposure to ammonium for 4d, content of O2(-) and H2O2 increased in leaves of H. verticillata exposed to 3mM ammonium compared with control (0mM NH4Cl), while the malondialdehyde content decreased. The chlorophyll (a+b) and carotenoid concentrations decreased in H. verticillata plants exposed to 1.5-3mM ammonium for 12h and 4d. Compared with controls, the activity of superoxide dismutase, peroxidase, catalase, glutathione reductase, ascorbate peroxidase, and glutathione S-transferase increased in plants treated with ammonium for 12h, and the activity of most enzymes was further enhanced at 4d. The changes in nonprotein thiols, total glutathione, ascorbic acid, and dehydroascorbate content were also assayed. Our results suggest that ammonium induced the oxidative stress and the heated antioxidant response in H. verticillata.
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