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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 19, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Rapid data analysis method for differential mobility cytometry.

Randall D Reif¹, Kelong Wang, Dimitri Pappas

¹Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.

Analytical and Bioanalytical Chemistry

|October 9, 2009

Summary

This summary is machine-generated.

Differential mobility cytometry (DMC) now offers improved cell adhesion analysis. A new movie subtraction technique enhances image processing, increasing throughput and accessibility for researchers studying cell adhesion.

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Area of Science:

Biophysics
Cell Biology
Biotechnology

Background:

Differential mobility cytometry (DMC) is a key technique for studying cell adhesion.
Current DMC methods face limitations in throughput due to manual image processing.

Purpose of the Study:

To introduce a novel, automated method for generating differential images in DMC.
To enhance the efficiency and accessibility of DMC for cell adhesion studies.

Main Methods:

A new image processing technique involving the subtraction of short video sequences.
Automated generation of a stack of differential images for analysis.

Main Results:

The developed method streamlines the creation of differential images.
The new approach significantly improves the throughput of DMC.

Conclusions:

This advancement makes differential mobility cytometry more accessible.
The improved throughput will accelerate research in cell adhesion processes.