[Research on piezoelectric protein sensor array for rapid detecting HIV(1+2) antibody]

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Immunodiagnostics

Context:

• Current methods for Human Immunodeficiency Virus (HIV) detection can be time-consuming.
• There is a need for rapid, sensitive, and specific diagnostic tools for HIV types 1 and 2.
• Piezoelectric biosensors offer potential for label-free and real-time detection.

Purpose:

• To develop and evaluate a piezoelectric immunosensor array for the rapid detection of HIV types 1 and 2.
• To immobilize HIV(1+2)C1 antigen onto silver electrodes of a quartz-crystal microbalance using adsorption and cross-linking.
• To assess the performance of the immunosensor array using standard quality control and clinical serum samples.

Summary:

• A piezoelectric immunosensor array matrix was designed for detecting HIV(1+2).
• HIV(1+2)C1 antigen was immobilized on silver electrodes of a quartz-crystal microbalance.
• The sensor demonstrated a linear range of 0.01-0.2 NCU/ml with sensitivity (91.7%), specificity (93.3%), and accuracy (92.7%) for HIV(1+2).

Impact:

• Provides a rapid and accurate diagnostic tool for HIV types 1 and 2.
• Potential for point-of-care testing and improved HIV screening.
• Contributes to advancements in piezoelectric biosensor applications for infectious disease detection.