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Updated: Jun 19, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

A multicenter blinded study to evaluate KRAS mutation testing methodologies in the clinical setting.

Vicki Whitehall¹, Kayla Tran, Aarti Umapathy

¹Queensland Institute of Medical Research, Bancroft Building, 300 Herston Road, Herston Queensland 4029, Australia. Vicki.Whitehall@qimr.edu.au

The Journal of Molecular Diagnostics : JMD

|October 10, 2009

Summary

This summary is machine-generated.

KRAS mutation testing is crucial for colorectal cancer treatment selection. This study found multiple detection methods provide comparable results for identifying these critical KRAS mutations.

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Area of Science:

Oncology
Molecular Diagnostics
Genetics

Background:

Activating KRAS oncogene mutations predict resistance to anti-epidermal growth factor receptor (EGFR) therapy in advanced colorectal cancer.
This necessitates routine KRAS mutation testing for personalized treatment selection.

Purpose of the Study:

To evaluate the concordance of six different KRAS mutation detection methodologies.
To assess their suitability for clinical application in colorectal cancer samples.

Main Methods:

DNA was extracted from 80 frozen and 74 formalin-fixed paraffin-embedded colorectal cancer samples.
KRAS mutations in codons 12 and 13 were analyzed using single strand conformation polymorphism, pyrosequencing, high resolution melting analysis, dideoxy sequencing, and two commercial kits.

Main Results:

Concordance (≥5 assays) in frozen samples was 83% (66/80).
Concordance in paraffin-embedded samples was 96% (71/74) using the top five assays, versus 63% (46/74) considering all six.

Conclusions:

Multiple KRAS mutation detection methodologies are suitable for clinical use.
These methods provide comparable results for KRAS mutation analysis in colorectal cancer specimens.