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Updated: Jun 19, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
Molecular weight and polydispersity of calf-thymus DNA: static light-scattering and size-exclusion chromatography
Bedrich Porsch1, Richard Laga, Jirí Horský
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. porsch@imc.cas.cz
Abstract:
Plausible calf-thymus DNA molecular weight distribution can be obtained by size-exclusion chromatography with dual low-angle light scattering/refractometric detection at sufficiently low flow rate. The distribution extends over three decades of molecular weight and is characterized by weight average molecular weight M(w) = 8418000 and polydispersity index M(w)/M(n) = 5.2. After strongly scattering impurities had been removed from the sample using adsorption properties of the 3 mum mixed-cellulose-ester filter membranes, static light-scattering measurement in flow injection mode was feasible and gave M(w) = 8580000, corroborating the veracity of SEC results.
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