Related Experiment Video
Updated: Jun 19, 2026
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Fabrication of chronocoulometric DNA sensor based on gold nanoparticles/poly(l-lysine) modified glassy carbon
Jie Wang1, Shaojun Zhang, Yuzhong Zhang
1College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China.
Abstract:
In this work, we fabricated a sensitivity chronocoulometric DNA sensor (CDS) based on gold nanoparticles (AuNPs)/poly(L-lysine) complex film modified glassy carbon electrode. Hexaammineruthenium(III) chloride ([Ru(NH3)6]3+) was used as the electroactive indicator. The assembled process was investigated by cyclic voltammetry (CV) and chronocoulometry (CC). CC is used to monitor the DNA hybridization event by measurement of electrostatic binding [Ru(NH3)6]3+. Under the optimal conditions, the signal of [Ru(NH3)6]3+ was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10(-13) to 1.0 x 10(-11) M, and the detection limit is 3.5 x 10(-14) M.
