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Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
The Bone Matrix01:18

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...

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Related Experiment Video

Updated: Jun 19, 2026

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
07:51

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Published on: March 18, 2019

Circulating fibronectin affects bone matrix, whereas osteoblast fibronectin modulates osteoblast function.

Anke Bentmann1, Nina Kawelke, David Moss

  • 1Max-Planck Institute for Biochemistry, 82152 Martinsried, Germany.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|October 14, 2009
PubMed
Summary
This summary is machine-generated.

Fibronectin in bone matrix is crucial for collagen integrity. Liver-derived fibronectin impacts bone matrix properties, while osteoblast-derived fibronectin influences osteoblast function, not mediated by RGD-binding integrins.

Related Experiment Videos

Last Updated: Jun 19, 2026

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
07:51

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Published on: March 18, 2019

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Biology

Background:

  • Fibronectin is essential for collagen matrix integrity in vitro.
  • Osteoblasts were previously assumed to be the primary source of fibronectin for bone matrix formation.

Purpose of the Study:

  • To investigate the distinct roles of osteoblast-derived and liver-derived fibronectin in bone matrix formation and integrity.
  • To identify the specific mechanisms and receptors involved in fibronectin's effects on osteoblasts.

Main Methods:

  • Utilized cre-loxP system in transgenic mice for conditional deletion of fibronectin in osteoblasts and liver.
  • Employed mice with mutated fibronectin and conditional deletion of beta(1)-integrin in osteoblasts.
  • Analyzed bone matrix composition, biomechanical properties, and cellular functions (osteoblasts, osteoclasts).

Main Results:

  • Conditional deletion of fibronectin in osteoblasts did not decrease bone matrix fibronectin levels but confirmed osteoblast-derived fibronectin affects osteoblast function, independent of RGD-motif integrins.
  • Conditional deletion of fibronectin in the liver significantly reduced bone matrix fibronectin, impaired mineral-to-matrix ratio, and altered biomechanical properties, without affecting osteoblast or osteoclast activity.
  • Liver-derived fibronectin plays a critical role in bone matrix quality.

Conclusions:

  • Osteoblast-derived fibronectin influences osteoblast function through non-RGD-mediated pathways.
  • Liver-derived circulating fibronectin is crucial for bone matrix properties, distinct from the role of locally produced fibronectin.
  • This study delineates novel functions for fibronectin originating from different sources in bone metabolism.