Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-loss reciprocal optical terminals for two-way time-frequency transfer.

Applied optics·2017
Same author

Invited Article: A compact optically coherent fiber frequency comb.

The Review of scientific instruments·2015
Same author

Comb-calibrated laser ranging for three-dimensional surface profiling with micrometer-level precision at a distance.

Optics express·2014
Same author

Operation of an optically coherent frequency comb outside the metrology lab.

Optics express·2014
Same author

Direct-comb molecular spectroscopy with accurate, resolved comb teeth over 43 THz.

Optics letters·2012
Same author

Multiply loaded magneto-optical trap.

Optics letters·2009

Related Experiment Video

Updated: Jun 19, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Coherent linear optical sampling at 15 bits of resolution.

I Coddington1, W C Swann, N R Newbury

  • 1National Institute of Standards and Technology, Boulder, CO 80305, USA. ian@nist.gov

Optics Letters
|October 14, 2009
PubMed
Summary

We developed a novel optical sampling technique using phase-locked lasers for high-fidelity measurements of optical electric fields. This method achieves exceptional signal-to-noise ratios and precise timing resolution for detailed sample characterization.

More Related Videos

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Jun 19, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Optics and Photonics
  • Laser Physics
  • Materials Science

Background:

  • Linear optical sampling quantifies sample properties by measuring optical field distortions.
  • High signal-to-noise ratio (SNR) is crucial for accurate optical response measurements.
  • Existing methods may face limitations in dynamic range or temporal resolution.

Purpose of the Study:

  • To introduce an advanced linear optical sampling technique.
  • To achieve high SNR measurements of transmitted optical electric fields.
  • To enhance the precision of optical characterization methods.

Main Methods:

  • Utilizing two phase-locked femtosecond fiber lasers.
  • Employing coherent averaging to boost signal-to-noise ratio.
  • Measuring transmitted optical electric fields with high dynamic range and timing resolution.

Main Results:

  • Achieved very high signal-to-noise ratio measurements.
  • Obtained 15.16 bits dynamic range (91 dB intensity).
  • Reached 525 fs timing resolution over a 10 ns window with 5.1 s averaging.

Conclusions:

  • The developed technique offers a significant improvement in optical electric field measurement.
  • High fidelity characterization of optical responses is now feasible.
  • This method advances precision in optical sampling and material analysis.