[Domestic chromogenic medium for isolation of Helicobacter pylori]

The aim of the investigation was to design a differentially diagnostic selective medium for accelerated isolation and identification of H. pylori, by using with erythrite-serum agar with urea and amphotericin (ESAM). The developed nutrient medium and three control ones: blood agar (BA), blood agar with amphotericin B at a concentration of 2 microg/ml (BAA) and erythrite-blood agar with urea and amphotericin (EBAA) were comparatively studied on 12 freshly isolated H. pylori and 15 biopsy specimens from patients with various gastroduodenal diseases. P. vulgaris 019 (a positive control) and E. coli 657 (a negative control) were used as a control of urease activity. ESAM was shown to isolate H. pylori from the biopsy specimens in 80% of cases. The investigations have indicated that the proposed medium isolates H. pylori at the level of control media and accelerates identification with the preservation of the major biological properties of the microorganism. Thus, erythrite-serum agar with urea may be recommended for use at practical laboratories for the diagnosis of H. pylori-associated infection diseases.