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Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas

Kazuhiro Daino1, Sandrine Roch-Lefevre, Nicolas Ugolin

  • 1LCE/iRCM/DSV/CEA, 92265 Fontenay-aux-Roses Cedex, France. k_daino@nirs.go.jp

Biochemical and Biophysical Research Communications
|October 15, 2009
PubMed

Insights

Radiation-induced osteosarcoma (OS) in rats shows down-regulation of Cited2 and Akap12 genes. These genes are identified as novel candidate tumor-suppressor genes in this specific cancer.

Area of Science:

  • Oncology
  • Genetics
  • Radiation Biology

Background:

  • Osteosarcoma (OS) is a bone cancer.
  • Radiation exposure can induce OS.
  • Previous studies identified gene expression changes in radiation-induced OS.

Purpose of the Study:

  • Investigate the role of Cited2 and Akap12 genes in radiation-induced rat osteosarcoma.
  • Determine if copy number losses correlate with gene silencing.
  • Evaluate Cited2 and Akap12 as potential tumor suppressors.

Main Methods:

  • Quantitative PCR (qPCR) to assess gene copy number.
  • Quantitative RT-PCR to measure mRNA expression levels.
  • Immunoblot analysis to detect protein expression.

Main Results:

  • Cited2 gene copy number loss observed in 53% of tumors.
  • Akap12 gene copy number loss observed in 67% of tumors.
  • Down-regulation of Cited2 and Akap12 mRNA and protein expression confirmed.

Conclusions:

  • Cited2 and Akap12 genes are frequently silenced in radiation-induced osteosarcoma.
  • These genes represent novel candidate tumor-suppressor genes for this cancer type.

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