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Updated: Jun 19, 2026

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

Plant cell wall matrix polysaccharide biosynthesis.

Ajay Pal S Sandhu¹, Gursharn S Randhawa, Kanwarpal S Dhugga

¹Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, 7300 NW 62nd Avenue, Johnston, IA 50131, USA.

Molecular Plant

|October 15, 2009

Summary

Plant cell expansion relies on Golgi-synthesized matrix polysaccharides. Recent advances identify genes for these enzymes, overcoming challenges in biochemical and genetic studies.

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Glycan Profiling of Plant Cell Wall Polymers using Microarrays

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Published on: December 17, 2012

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Published on: June 20, 2010

Area of Science:

Plant Biology
Cell Biology
Biochemistry

Background:

Plant cell walls are crucial for expansion, composed of cellulose microfibrils and matrix polysaccharides.
Matrix polysaccharides are synthesized in the Golgi apparatus and secreted via exocytosis.
The genes for Golgi-localized glucan synthases, essential for cell expansion, were recently identified.

Purpose of the Study:

To identify and functionally characterize genes encoding Golgi-localized glucan synthases involved in plant cell wall biosynthesis.
To overcome limitations of traditional biochemical and mutational approaches in studying these enzymes.

Main Methods:

Gene expression profiling and comparative genomics to identify candidate genes.
Heterologous expression systems for functional characterization of identified genes.
Analysis of cellulose synthase-like (Csl) gene family members.

Main Results:

Several cellulose synthase-like (Csl) genes have been identified as crucial for synthesizing various hemicellulosic glycans.
Functional characterization in heterologous systems proved successful for several candidate genes.
The study highlights the effectiveness of gene expression and genomics approaches.

Conclusions:

Gene expression profiling and heterologous expression are powerful tools for dissecting Golgi polysaccharide biosynthesis.
The cellulose synthase-like (Csl) family plays a significant role in hemicellulose synthesis.
Further research using nonconventional approaches is needed to fully elucidate Golgi polysaccharide biosynthesis mechanisms.