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Updated: Jun 19, 2026
An Efficient Method for Adenovirus Production
Published on: June 10, 2021
In vitro cytotoxic effects of benzalkonium chloride on adenovirus
Douglas R Lazzaro1, Khaled Abulawi, Mohammedyusuf E Hajee
1Departments of Ophthalmology, SUNY-Downstate Medical Center, Kings County Hospital Center, Brooklyn, NY, USA. drleyedoc@aol.com
Objective:
To investigate the in vitro effects of benzalkonium chloride (BAK) at various concentrations and dosing schedules on adenoviral infection.
Methods:
Adenovirus was placed in Refeed media and at 30 min after infection, various concentrations and doses of BAK were placed into the 1 mL solution. All assays were incubated at 37 degrees C. Dosing of BAK was performed for 3 days and qualitative viral immunofluorescence was compared with that of controls.
Results:
BAK at multiple concentrations lysed the epithelial cell lines to varying degrees as did viral infection. Further, adenoviral infection was halted with many concentrations as well.
Conclusions:
Adenoviral infection was inhibited in vitro by different concentrations of BAK.
Clinical Relevance:
Zymar solution which contains BAK could potentially have a role in the treatment of adenoviral ocular infection in humans.
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