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17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
The adaptor protein Shc plays a key role during early B cell development
Amber J Giles1, Timothy P Bender, Kodi S Ravichandran
1Carter Immunology Center and Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|October 16, 2009
Summary
The adaptor protein Shc is crucial for early B cell development, particularly for survival signals from IL-7. Loss of Shc leads to increased cell death during B lymphopoiesis.
Area of Science:
- Immunology
- Cell Biology
- Developmental Biology
Background:
- The adaptor protein Shc is activated by various cell surface receptors.
- The function of Shc in B cell development remains largely unknown.
Purpose of the Study:
- To investigate the role of the adaptor protein Shc in early B cell development (B lymphopoiesis).
Main Methods:
- Conditional expression of a dominant-negative Shc mutant.
- Conditional loss of Shc protein expression in mice.
- Analysis of B cell populations and apoptosis.
Main Results:
- Shc is required for B cell development starting at the pre-pro-B to pro-B stage transition.
- Impaired Shc signaling results in increased apoptosis and reduced pre-B cell numbers.
- Shc is phosphorylated upon IL-7 stimulation and plays a role in IL-7-dependent signaling in pro-B cells.
Conclusions:
- Shc is critical for early B lymphopoiesis, specifically for the survival of developing B cells.
- Shc acts as a key signaling molecule downstream of the IL-7 receptor in early B cells.
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