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A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
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Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation

P Montreekachon1, P Chotjumlong, V Reutrakul

  • 1Center of Excellence for Innovation in Chemistry, Department of Odontology and Oral Pathology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand.

Journal of Dental Research
|October 16, 2009
PubMed

Insights

Cytosolic phospholipase A2alpha (cPLA2alpha) regulates matrix metalloproteinase-9 (MMP-9) in gingival cells. Inhibition of cPLA2alpha significantly reduced MMP-9 induction by Fusobacterium nucleatum and PMA, highlighting its role in periodontitis pathogenesis.

Area of Science:

  • Oral Biology
  • Molecular Biology
  • Enzymology

Background:

  • Matrix metalloproteinase-9 (MMP-9) plays a key role in periodontitis pathogenesis.
  • Cytosolic phospholipase A2alpha (cPLA2alpha) is known to up-regulate MMP-9 in human monocytes.

Purpose of the Study:

  • To investigate the role of cPLA2alpha in MMP-9 induction by Fusobacterium nucleatum and phorbol 12-myristate-13-acetate (PMA) in gingival epithelial cells.

Main Methods:

  • Time-course study of MMP-9 mRNA and protein expression and activity.
  • Assessment of cPLA2alpha activation via phosphorylation.
  • Inhibition of cPLA2alpha using a specific inhibitor.

Main Results:

  • PMA and F. nucleatum induced MMP-9 expression and activity in gingival epithelial cells.
  • MMP-9 mRNA levels increased at 3 hours, while secretion and activity peaked at 12 hours.
  • cPLA2alpha activation, indicated by Ser505 phosphorylation in the nucleus, correlated with MMP-9 induction.
  • A specific cPLA2alpha inhibitor significantly reduced MMP-9 induction (P < 0.01).

Conclusions:

  • cPLA2alpha is involved in the up-regulation of MMP-9 in gingival epithelial cells.
  • These findings suggest cPLA2alpha as a potential therapeutic target in periodontitis.

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