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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Difference from Background: Limit of Detection

Difference from Background: Limit of Detection

The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...

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Related Experiment Video

Updated: Jun 19, 2026

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Published on: May 19, 2023

[Method for estimating the precision and detection limit for ELISA]

Kazuo Iwaki¹, Yuzuru Hayashi

¹k-iwaki@pha.ohu-u.ac.jp

Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica

|October 16, 2009

Summary

No abstract available in PubMed .

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