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An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
Published on: September 12, 2019
[Tumor associated antigen CHP2 promotes intraperitoneal metastasis of HEK293 cells in nude mice]
Xiao Ping Qian1, Xiu Yuan Sun, Guo Dong Li
1T Cell Laboratory, Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Objective:
To investigate the effect of tumor associate antigen of CHP2 on the metastatic potential of HEK293 cells in nude mice.
Methods:
Matrigel testing by Boyden chamber and intraperitoneal inoculation of BALB/c nude mice were respectively performed to detect the metastatic potential of HEK293 transfectants in vitro and in vivo. HE staining was used to confirm the tumor metastasis in related organs. Molecules probably involved in metastasis of HEK293 cells were screened by RT-PCR.
Results:
CHP2 significantly promoted the migration of HEK293 cells through matrigel in vitro. CHP2 accelerated the tumorigenesis of HEK293 cells in nude mice and tissue invasion was observed in spleen, kidney and liver. CHP2 up-regulated osteopontin (OPN) expression in HEK293 cells, but had no effect on MMP2 expression.
Conclusion:
CHP2 enhanced the migration and metastasis of HEK293 cells in vitro and in vivo. Up-regulated OPN expression might contribute to the invasive potential of HEK293 cells.
