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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 19, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

Proteomics on Fixed Tissue Specimens - A Review.

Beth Ann Reimel¹, Sheng Pan, Damon H May

¹Departments of Gastroenterology and Surgery, University of Washington, Seattle, Washington 98195.

Current Proteomics

|October 16, 2009

Summary

This summary is machine-generated.

Formalin-fixed, paraffin-preserved clinical tissues are now accessible for proteomic analysis. New methods efficiently extract proteins from these valuable archived samples, enabling clinical research translation.

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

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Last Updated: Jun 19, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Area of Science:

Biochemistry
Proteomics
Histology

Background:

Formalin-fixed, paraffin-preserved (FFPE) tissues are standard for clinical archiving.
FFPE preservation poses challenges for protein extraction and proteomic analysis.
Archived FFPE tissues contain crucial patient data for translational research.

Purpose of the Study:

To review the evolution of techniques for protein extraction from FFPE tissues.
To summarize proteomic findings from FFPE samples.
To discuss the implications of these advancements for clinical proteomics.

Main Methods:

Review of published literature on FFPE protein extraction techniques.
Analysis of proteomic studies utilizing FFPE samples.
Discussion of method development and application in clinical proteomics.

Main Results:

Development of efficient methods to reverse protein cross-linking in FFPE tissues.
Successful application of these methods in various clinical proteomic studies.
Identification of key proteomic signatures in archived tissues.

Conclusions:

Protein extraction from FFPE tissues is now feasible, overcoming previous limitations.
These advancements significantly enhance the utility of archived clinical samples for proteomic research.
Future clinical proteomics studies can leverage FFPE samples for translational discoveries.