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Updated: Jun 19, 2026

Synthesis of Esters Via a Greener Steglich Esterification in Acetonitrile
Published on: October 30, 2018
Enzymatic production of linalool esters in organic and solvent-free system
Natália Paroul1, Adriana Biasi, Ane Cheila Rovani
1Programa de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil.
Abstract:
This work investigated the influence of temperature, enzyme concentration, substrates molar ratio, in the absence and presence of organic solvent, at two molar ratios of the substrates on the enzymatic production of linalil esters using the immobilized lipase Novozym 435 as catalyst, different acids and linalool and Ho-Sho essential oil as substrates. The best reaction conversion was obtained at the highest temperature (70 degrees C), for both solvent free (3.81%) and with solvent addition (2.25%), for a solvent to substrates molar ratio of 2:1, enzyme concentration of 5 wt% and acid to alcohol molar ratio of 1:1. The reaction kinetics revealed that Ho-Sho essential oil afforded the greatest conversions when compared with pure linalool. Higher linalil esters production were achieved after 10 h reaction (5.58%) in 2:1 solvent to substrates molar ratio, with enzyme concentration of 5 wt%, at 70 degrees C and anhydride to alcohol molar ratio of 1:1 using Ho-Sho essential oil as substrate.
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