Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Theory of Strong Electrolytes01:23

Theory of Strong Electrolytes

The interionic forces of the strong electrolytes depend on the solvent's dielectric constant, which is the ability of a solvent to store electrical energy, based on its polarizability. and the solution's concentration. In high-dielectric solvents and in dilute solutions, weak electrostatic forces keep ions apart. However, in low-dielectric solvents or concentrated solutions, stronger interionic forces may cause ions to pair up as ionic doublets despite being fully ionized. The theory of strong...
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
Determination of Molar Masses of Polymers II01:27

Determination of Molar Masses of Polymers II

Polymer samples typically consist of macromolecular chains with a distribution of lengths, resulting in a range of molar masses rather than a single discrete value. Conventional descriptors such as the number-average molar mass and weight-average molar mass quantify this distribution but do not fully capture polymer behavior in solution..The viscosity-average molar mass provides a more realistic description of polymer behavior in solution because it accounts for the enhanced contribution of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Success of Dental Implant Placed in Ridge with Inadequate Bone Width and Exposed Screw Threads-A CBCT Case Report with 8 Years Follow Up.

Journal of pharmacy & bioallied sciences·2026
Same author

Development of cellulose and banana bract extract reinforced poly(vinyl alcohol) film incorporated with in-situ generated nano silver as freshness indicating packaging film.

International journal of biological macromolecules·2025
Same author

Posterior Circulation Stroke Due to Arterial Thoracic Outlet Syndrome: A Case Report and Literature Review.

Annals of Indian Academy of Neurology·2024
Same author

Development of banana pseudo stem cellulose fiber based magnetic nanocomposite as an adsorbent for dye removal.

International journal of biological macromolecules·2024
Same author

Bioderived cellulose fibre-guar gum grafted poly (N, N'-dimethylacrylamide) polymer network for controlled release of metformin hydrochloride.

International journal of biological macromolecules·2023
Same author

Graphene oxide functionalized chitosan-magnetite nanocomposite for removal of Cu(II) and Cr(VI) from waste water.

International journal of biological macromolecules·2020
Same journal

A chemiluminescence sensor for ciprofloxacin detection based on copper ion and aptamer co-modified magnetic microspheres.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Application of far-infrared spectroscopy for prediction of silicate mineral content in claystones and clay shales.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

A coumarin-based water-soluble fluorescent probe for tandem detection of Cu<sup>2+</sup> and glutathione with application in bioimaging and real sample analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Chromone-based thiosemicarbazone and semicarbazone as efficient fluorescent sensors for CrO<sub>4</sub><sup>2-</sup>/ Cr<sub>2</sub>O<sub>7</sub><sup>2-</sup> Ion detection.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Rapid species authentication and protein prediction of porcini mushrooms using FTIR-2DCOS coupled with deep learning.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Synthesis of a cyanine-based fluorescent probe and its dual-channel recognition of TNP and PYX in aqueous solution.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

A comparative study of polyelectrolyte-dye interactions.

R Nandini1, B Vishalakshi

  • 1Department of Chemistry, MITE, Moodabidri 572226 (DK), Karnataka, India. nandinifalnir@yahoo.com

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 17, 2009
PubMed
Summary
This summary is machine-generated.

Azure B forms metachromatic complexes with sodium alginate and heparin. Sodium alginate more effectively reduces Azure B absorbance, indicating stronger electrostatic interactions due to its higher negative charge.

More Related Videos

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
08:41

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

Related Experiment Videos

Last Updated: Jun 19, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
08:41

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Azure B is a thiazine dye used in biological staining.
  • Sodium alginate and heparin are polysaccharides with significant biological and industrial applications.
  • Understanding dye-polymer interactions is crucial for developing new analytical and material science applications.

Purpose of the Study:

  • To investigate the spectrophotometric interaction of Azure B with sodium alginate and heparin in aqueous solutions.
  • To determine the stoichiometry and nature of the complexes formed.
  • To evaluate the thermodynamic stability and factors influencing the metachromatic complex formation.

Main Methods:

  • Spectrophotometry was employed to monitor changes in Azure B absorbance.
  • The stoichiometry of the complexes was determined using spectrophotometric titration.
  • The influence of alcohol, urea, and surfactants on the interaction was studied.

Main Results:

  • Azure B exhibited metachromasy upon interaction with sodium alginate and heparin, forming new complexes with distinct spectral bands.
  • Sodium alginate demonstrated a greater ability to decrease Azure B absorbance compared to heparin.
  • Electrostatic forces were identified as the primary interaction mechanism, with charge density differences explaining variations between the two polysaccharides.

Conclusions:

  • Sodium alginate and heparin interact with Azure B aggregates through electrostatic forces.
  • The differential effectiveness of sodium alginate and heparin is attributed to their distinct negative charge densities.
  • Thermodynamic analysis confirmed the stability of the metachromatic complexes, and surfactant effects on metachromasy were elucidated.