Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.6K
No description available
3.6K
Transcription01:17

Transcription

34.2K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
34.2K
Transcription01:10

Transcription

157.6K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A designed overlapping variant immunogen pool elicits broad sarbecovirus neutralization.

bioRxiv : the preprint server for biology·2026
Same author

Seasonal vaccine-induced immunity shows preserved cross-reactivity to H3N2 subclade K in adults.

EBioMedicine·2026
Same author

Seabirds' mortalities in Antarctica caused by HPAIV: Skuas as a sentinel species.

Epidemiology and infection·2026
Same author

Early immune events during SARS-CoV-2 infection impact memory T and B cell responses.

Communications biology·2026
Same author

Characterizing acute and persistent symptoms of COVID-19 among adults and children in rural Zambia, 2020-2022.

PLOS global public health·2026
Same author

Coronavirus protein interaction mapping in bat and human cells reveals network rewiring governing immune evasion and zoonotic potential.

Cell host & microbe·2026
Same journal

From consensus structure prediction to RNA gene finding.

Briefings in functional genomics & proteomics·2009
Same journal

The use of covariance models to annotate RNAs in whole genomes.

Briefings in functional genomics & proteomics·2009
Same journal

Annotating non-coding transcription using functional genomics strategies.

Briefings in functional genomics & proteomics·2009
Same journal

The role of RNA molecules in cellular biology. Editorial.

Briefings in functional genomics & proteomics·2009
Same journal

Sequencing genomes: from individuals to populations.

Briefings in functional genomics & proteomics·2009
Same journal

Insights into the nature and consequences of our variable genome.

Briefings in functional genomics & proteomics·2009
See all related articles

Related Experiment Video

Updated: Feb 27, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

443

Establishing legitimacy and function in the new transcriptome.

Harm van Bakel1, Timothy R Hughes

  • 1Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada.

Briefings in Functional Genomics & Proteomics
|October 17, 2009
PubMed
Summary
This summary is machine-generated.

The discovery of novel non-coding RNAs (ncRNAs) is rapidly advancing, but validating their function and existence presents significant challenges for researchers. This review explores the hurdles in identifying and characterizing these emerging ncRNA classes.

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.2K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.7K

Related Experiment Videos

Last Updated: Feb 27, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

443
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.2K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Recent years have witnessed a surge in the identification of novel non-coding RNA (ncRNA) molecules.
  • While some ncRNAs are recognized as functional, others lack definitive evidence for their roles or even stable existence.

Purpose of the Study:

  • To discuss the challenges inherent in the discovery and characterization of non-traditional ncRNA categories.
  • To highlight the current state of research in ncRNA identification and functional validation.

Main Methods:

  • Literature review of recent advancements in ncRNA research.
  • Analysis of methodologies used for ncRNA discovery and characterization.
  • Discussion of criteria for establishing functional ncRNAs.

Main Results:

  • The rapid discovery of ncRNAs outpaces robust validation methods.
  • Defining the functional relevance and existence of novel ncRNAs remains a significant hurdle.
  • Distinguishing true functional ncRNAs from transcriptional noise is complex.

Conclusions:

  • Establishing the validity of new ncRNA classes requires rigorous experimental evidence.
  • Standardized criteria are needed for the classification and validation of novel ncRNAs.
  • Overcoming discovery and characterization challenges is crucial for advancing RNA biology.