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Alternatively spliced Hox-1.7 transcripts encode different protein products.

M R Rubin1, M C Nguyen-Huu

  • 1Department of Microbiology, Columbia University, New York, New York 10032.

DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1990
PubMed
Summary
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Guinea-pig kidney cells express two Hox-1.7 complementary DNAs (cDNAs) through alternative splicing. This process, involving a splice site upstream of the homeobox, generates distinct protein products from the Hox-1.7 gene.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Hox genes are crucial for embryonic development.
  • Hox-1.7 is a homeobox-containing gene involved in developmental patterning.
  • Understanding gene regulation and alternative splicing is key to comprehending protein diversity.

Purpose of the Study:

  • To isolate and characterize Hox-1.7 complementary DNAs (cDNAs) from guinea-pig kidney.
  • To investigate the molecular mechanisms underlying Hox-1.7 gene expression in guinea pigs.
  • To determine if alternative splicing contributes to Hox-1.7 transcript diversity.

Main Methods:

  • Isolation of guinea-pig kidney cDNAs using a mouse Hox-1.7 cDNA probe via low-stringency hybridization.
  • DNA sequencing of isolated cDNAs (GPK5 and GPK6).

Related Experiment Videos

  • Sequence comparison with known mouse Hox-1.7 cDNA (MH-1).
  • Southern blot analysis to confirm gene structure and splicing patterns.

    • Main Results:

    • Two distinct Hox-1.7 cDNAs, GPK5 and GPK6, were identified in adult guinea-pig kidney.
    • GPK5 potentially represents a full-length coding sequence.
    • GPK6 shares high amino acid identity with mouse Hox-1.7 (MH-1).
    • Both guinea-pig cDNAs possess identical homeodomains to mouse Hox-1.7.
    • Differences in coding sequences upstream of the homeobox suggest alternative splicing.
    • Southern blot analysis supports the presence of a splice site 5' to the homeobox.

    Conclusions:

    • Alternative splicing of the Hox-1.7 gene in guinea pigs generates at least two different transcript variants.
    • These variants encode distinct protein products due to variations upstream of the homeobox.
    • The findings highlight the role of alternative splicing in expanding the functional repertoire of Hox genes.