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Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

Strategies for single particle manipulation using acoustic and flow fields.

S Oberti¹, D Möller, A Neild

¹Institute of Mechanical Systems, Dept. of Mechanical and Process Eng., ETH Zurich, CH-8092 Zurich, Switzerland. stefano.oberti@imes.mavt.ethz.ch

|October 20, 2009

Summary

Acoustic radiation forces enable simultaneous positioning of micrometer-sized particles in fluidic channels for automated single-particle manipulation. This technique facilitates pre-alignment for subsequent removal and analysis, enhancing research efficiency.

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Area of Science:

Acoustic manipulation
Microfluidics
Biotechnology

Background:

Acoustic radiation forces are effective for manipulating microparticles.
Acoustic standing wave fields offer simultaneous particle manipulation throughout fluidic volumes.

Purpose of the Study:

To exploit simultaneous positioning for pre-aligning particles in microchannels.
To enable automated single-particle manipulation and analysis.
To demonstrate applications in microgripping and crystallographic sample preparation.

Main Methods:

Utilizing acoustic standing wave fields for particle manipulation.
Employing microfluidic channels for particle guidance.
Integrating acoustic manipulation with microgrippers and laminar flow for specific applications.

Main Results:

Particles are successfully pre-aligned along channel centerlines.
Automation of single-particle manipulation is achieved by eliminating the need for initial location identification.
Demonstrated applications include copolymer bead and cell manipulation, and crystal positioning for crystallographic analysis.

Conclusions:

Acoustic radiation forces provide a robust method for automated particle manipulation in microfluidics.
The technique enhances efficiency in single-particle analysis and sample preparation for crystallography.
Simultaneous positioning capability is key to achieving predetermined particle arrangements.