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Updated: Jun 19, 2026
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A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
Published on: September 14, 2011
Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue
James Carroll1, Pierre Douarre, Aidan Coffey
1Department of Biological Science, Cork Institute of Technology, Cork, Ireland.
Applied and Environmental Microbiology
|October 20, 2009
Abstract:
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.
