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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

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Published on: March 9, 2017

Quantitative biomarker assay with microfluidic paper-based analytical devices.

Xu Li1, Junfei Tian, Wei Shen

  • 1Australian Pulp and Paper Institute, Department of Chemical Engineering, Monash University, Clayton, Melbourne, 3800, Victoria, Australia.

Analytical and Bioanalytical Chemistry
|October 20, 2009
PubMed
Summary

Microfluidic paper-based analytical devices (muPADs) enable cost-effective quantitative chemical analysis. This method minimizes errors in colorimetric assays by simultaneously measuring standards and samples on a single device.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (muPADs) are suitable for colorimetric biochemical assays.
  • Paper background color variations and measurement device inaccuracies can introduce errors.
  • Existing methods may struggle with consistent quantitative results due to these error sources.

Purpose of the Study:

  • To develop a method for accurate quantitative chemical assays using muPADs.
  • To minimize errors in colorimetric measurements on paper-based devices.
  • To establish a cost-effective analytical approach for chemical assays.

Main Methods:

  • Utilizing muPADs with multiple detection zones for simultaneous assays.
  • Performing quantitative chemical assays with internal standards.
  • Establishing analyte concentration calibration curves from simultaneously measured standards and samples.

Main Results:

  • Minimized errors from paper background and measurement devices.
  • Simultaneous measurement of standards and samples under identical conditions.
  • Demonstrated the feasibility of accurate quantitative analysis using muPADs.

Conclusions:

  • muPADs can perform reliable quantitative chemical analysis.
  • The developed method significantly reduces errors in colorimetric assays.
  • This approach offers a very low-cost solution for chemical analysis.