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Updated: Jun 19, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
Published on: October 19, 2015
[Study on the pH-sensitive secondary structure of gamma-PGA embedded with magnetite nanoparticles]
Ju-Hua Zhang1, Jian-Min Xing, Yang-Yang Jiang
1Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. zhangjuhua05@yahoo.com.cn
Abstract:
The conformational transition of poly gamma-glutamic acid (gamma-PGA) embedded with magnetite nanoparticles under various pH conditions was investigated by Fourier transform infrared spectroscopy (FTIR). The secondary structure content was determined through the analysis of amide I bands of Fourier deconvolution spectra, secondary derivative spectra and the Gaussian curve fitting of the original infrared spectra. The results showed that the conformation of the gamma-PGA was affected by solution pH. The total contents of beta-sheet and beta-turn were higher than 65%, while alpha-helix and random coil were low. The content of beta-turn increased with increasing pH, while the beta-sheet decreased. Additionally, the zeta potential results showed that the pH-sensitive secondary structure of gamma-PGA had influence on the stability of suspension of magnetic gamma-PGA nanospheres. The minimum value of zeta potential (-35. 4 mV) was obtained at pH 10.2.
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