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Osteoclasts in Bone Remodeling

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Related Experiment Video

Updated: Jun 19, 2026

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

Propolis inhibits osteoclast maturation.

Roberta Pileggi1, Kathryn Antony2, Kristie Johnson2

  • 1Department of Endodontics, University of Florida, College of Dentistry, FL, USA.

Dental Traumatology : Official Publication of International Association for Dental Traumatology
|October 22, 2009
PubMed
Summary

Propolis, a natural bee product, significantly reduces osteoclast maturation, inhibiting bone resorption. This suggests propolis may be a valuable treatment for tooth injuries, preventing complications like inflammatory resorption.

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Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
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Published on: May 19, 2019

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Last Updated: Jun 19, 2026

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
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Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Area of Science:

  • Biomaterials Science
  • Dental Research
  • Pharmacology

Background:

  • Propolis, a natural resinous mixture collected by honey bees, possesses known anti-inflammatory and antimicrobial properties.
  • Traumatic dental injuries, particularly avulsion, can lead to challenging post-treatment complications such as inflammatory and replacement resorption.
  • Reducing osteoclast activity is a potential therapeutic strategy for managing resorption following dental trauma.

Purpose of the Study:

  • To investigate the potential of propolis as an anti-resorptive agent.
  • To evaluate propolis's effect on osteoclast formation and activity in vitro.

Main Methods:

  • Calcitriol-stimulated mouse marrow cultures containing osteoblasts and osteoclasts were treated with ethanol extracts of propolis.
  • Osteoclasts were identified and quantified by staining for tartrate-resistant acid phosphatase (TRAP)-activity.
  • RAW 264.7 osteoclast-like cells were used to assess propolis's direct effects on osteoclast maturation, including actin ring formation.

Main Results:

  • Propolis demonstrated a significant, dose-dependent reduction in multinuclear TRAP+ osteoclasts.
  • Propolis treatment supported overall cell growth and survival.
  • Propolis inhibited the formation of actin rings in osteoclast-like cells, indicating direct action on maturation.

Conclusions:

  • Propolis effectively inhibits late-stage osteoclast maturation, including cell fusion and actin ring formation.
  • These findings support the hypothesis that propolis may serve as a beneficial medicament to mitigate bone resorption associated with traumatic dental injuries.