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Double-stranded RNA poly(I:C) enhances matrix metalloproteinase mRNA expression in human nasal polyp epithelial cells

Jiyun Wang1, So Watanabe, Satoshi Matsukura

  • 1Department of Otorhinolaryngology, Showa University School of Medicine, Tokyo, Japan.

Acta Oto-Laryngologica. Supplementum
|October 24, 2009
PubMed
Abstract

Insights

Viral infection significantly increases matrix metalloproteinase (MMP)-9 mRNA in upper airways. This heightened proteolytic activity may worsen chronic rhinosinusitis with nasal polyps.

Area of Science:

  • Otolaryngology
  • Immunology
  • Molecular Biology

Background:

  • Chronic rhinosinusitis (CRS) is frequently exacerbated by viral infections.
  • Viral infections may disrupt the regulation of matrix metalloproteinases (MMPs), contributing to CRS exacerbation.
  • Understanding MMP expression in nasal epithelium during viral infection is crucial.

Purpose of the Study:

  • To investigate the expression of MMP-2, MMP-9, and TIMP-1 mRNA in nasal polyp epithelial cells following viral stimulation.
  • To elucidate the role of MMP dysregulation in viral-induced exacerbation of chronic rhinosinusitis.

Main Methods:

  • Primary human nasal polyp epithelial cells were isolated from CRS patients.
  • Cells were stimulated with dsRNA to mimic viral infection.
  • Expression levels of MMP-2, MMP-9, and TIMP-1 mRNA were quantified.

Main Results:

  • Only MMP-9 mRNA expression was significantly upregulated after dsRNA stimulation.
  • TIMP-1 expression did not show significant modulation in response to dsRNA.

Conclusions:

  • Significant upregulation of MMP-9 mRNA occurs in virus-infected upper airway epithelial cells.
  • This increased MMP-9 contributes to elevated proteolytic activity.
  • This may exacerbate chronic rhinosinusitis with nasal polyps.

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