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Related Experiment Video

Updated: Jun 19, 2026

Synthesis and Characterization of Supramolecular Colloids

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

Cationic vesicles from novel bolaamphiphilic compounds.

Mary Popov¹, Charles Linder, Richard J Deckelbaum

¹Department of Clinical Biochemistry, Ben-Gurion University, Beer Sheva, Israel.

Journal of Liposome Research

|October 24, 2009

Summary

Bolaamphiphiles with specific ester amide structures form stable nanovesicles for improved drug delivery. These stable vesicles demonstrated excellent stability in circulation, overcoming limitations of traditional cationic liposomes.

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Published on: April 8, 2020

Area of Science:

Biomaterials Science
Nanotechnology
Drug Delivery Systems

Background:

Cationic liposomes face challenges in targeted drug delivery due to rapid blood clearance.
Bolaamphiphiles forming monolayer membranes offer potential for enhanced vesicle stability, inspired by archaeosomes.

Purpose of the Study:

To investigate bolaamphiphiles with acetylcholine head groups and varied hydrophobic domains for stable nanovesicle formation.
To correlate bolaamphiphile structure with nanovesicle stability in vitro and in vivo for drug delivery applications.

Main Methods:

Synthesized and characterized a series of bolaamphiphiles with systematic structural modifications in the hydrophobic domain.
Formed nanovesicles and assessed their stability in phosphate-buffered saline (PBS) and mouse blood circulation.
Evaluated vesicle size, morphology, and drug encapsulation retention over time.

Main Results:

Bolaamphiphiles with short amide midsections formed unstable nanovesicles (80-120 nm) that lost payload within 24 hours in PBS.
Longer midsections yielded mixed fibers and more stable nanovesicles, while ester amide midsections produced highly stable spherical nanovesicles in PBS for days.
Vesicles with acetylcholine groups linked via the acetyl group were more stable than those linked via the amine.
In vitro stable vesicles exhibited good stability in mouse blood circulation post-intravenous administration.

Conclusions:

Bolaamphiphile structure, particularly the ester amide midsection, is critical for forming stable cationic nanovesicles.
Stable nanovesicles derived from specific bolaamphiphiles show promise for overcoming clearance issues in targeted drug delivery.
Findings provide structural insights for designing effective bolaamphiphile-based drug delivery systems.