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Phage displayed scFv: pIII scaffold may fine tune binding specificity

Pooja Goswami1, Deepti Saini, Subrata Sinha

  • 1Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.

Hybridoma (2005)
|October 28, 2009
PubMed
Summary

Antibody specificity is crucial for diagnostics and therapy. This study found that a phage-displayed antibody clone (E6) lost its specific binding to placental alkaline phosphatase (PLAP) when reformatted as a soluble scFv, suggesting scaffold influence.

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