Related Experiment Video
Updated: Jun 19, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): gene, inductive expression pattern
Yan Shi1, Zhe Zhao, Jing-Kui Yin
1Pearl River Fishery Research Institute, Chinese Academic of Fishery Science, Guangzhou, 510380, China.
Researchers identified the Interferon Regulatory Factor 2 (IRF-2) gene in orange-spotted grouper. This gene is widely expressed in fish tissues and its expression increases when challenged with immune stimulants, suggesting a role in fish immunity.
Area of Science:
- Molecular Biology
- Immunology
- Aquaculture
Background:
- Interferon regulatory factor 2 (IRF-2) is a key transcription factor involved in immune responses.
- Understanding IRF-2 in fish is crucial for aquaculture health and disease resistance.
Purpose of the Study:
- To clone and characterize the IRF-2 gene from orange-spotted grouper (Epinephelus coioides).
- To investigate the expression patterns and subcellular localization of EcIRF-2.
Main Methods:
- Cloning of the full-length cDNA and genomic DNA of EcIRF-2.
- Gene expression analysis in various tissues using quantitative methods.
- Subcellular localization studies in fish cells.
Main Results:
- The 1854bp cDNA sequence of EcIRF-2 was successfully cloned, encoding a 336-amino acid peptide.
- EcIRF-2 is ubiquitously expressed in healthy grouper tissues.
- Expression of EcIRF-2 was significantly upregulated in response to poly I:C and LPS challenges.
- EcIRF-2 was localized to the nucleus of fish cells.
Conclusions:
- This study reports the first characterization and localization of fish IRF-2.
- EcIRF-2 plays a role in the immune response of orange-spotted grouper.
- Provides a foundation for further research into IRF family functions in fish immunity.
More Related Videos
08:37Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
Published on: June 25, 2019
09:31Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Published on: January 12, 2015
Related Concept Videos
Reporter Genes
Commonly used reporter...
Cis-regulatory Sequences
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...