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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 19, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
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Kerr-lens mode-locked diode-pumped Cr:LiSGAF laser.

V P Yanovsky, F W Wise, A Cassanho

    Optics Letters
    |October 28, 2009
    PubMed
    Summary

    We demonstrated stable, self-sustaining Kerr-lens mode locking in a Cr:LiSGAF laser using AlGaInP laser diodes. This all-solid-state system produces 100-fs pulses tunable from 810-860 nm with low power fluctuations.

    Area of Science:

    • Laser Physics
    • Solid-State Lasers

    Background:

    • Cr:LiSGAF lasers offer potential for tunable ultrashort pulse generation.
    • AlGaInP laser diodes provide a compact and efficient pumping source.

    Purpose of the Study:

    • To demonstrate stable and self-sustaining Kerr-lens mode locking in a Cr:LiSGAF laser.
    • To characterize the performance of such a laser system.

    Main Methods:

    • Utilized a Cr:LiSGAF crystal as the gain medium.
    • Employed AlGaInP laser diodes for optical pumping.
    • Implemented Kerr-lens mode locking with a hard aperture for amplitude modulation.

    Main Results:

    • Achieved stable and self-sustaining Kerr-lens mode locking.
    • Generated transform-limited pulses with a duration of 100 femtoseconds (fs).

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  • Demonstrated tunability of the output pulses from 810 to 860 nanometers (nm).
  • Obtained an average output power of 35 milliwatts (mW) at 830 nm with minimal power fluctuations (<1%).
  • Conclusions:

    • The demonstrated all-solid-state system is capable of producing high-quality ultrashort laser pulses.
    • The thermal properties of Cr:LiSGAF suggest potential for power scaling.
    • This work paves the way for practical applications requiring tunable femtosecond lasers.