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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
The Central Dogma01:25

The Central Dogma

Overview
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

RNA-seq: from technology to biology.

Samuel Marguerat1, Jürg Bähler

  • 1Department of Genetics, Evolution and Environment, UCL Cancer Institute, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK.

Cellular and Molecular Life Sciences : CMLS
|October 28, 2009
PubMed
Summary
This summary is machine-generated.

RNA-sequencing (RNA-seq) analyzes dynamic transcriptomes, offering new insights into gene expression and regulation. This technology reveals complex transcript structures and processing with high resolution.

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AQRNA-seq for Quantifying Small RNAs
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AQRNA-seq for Quantifying Small RNAs

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) enables transcriptome analysis via RNA-sequencing (RNA-seq).
  • RNA-seq offers a dynamic view of genome expression, complementing static genome analysis.

Purpose of the Study:

  • To describe technical aspects of RNA-seq data generation and analysis.
  • To review biological insights gained from RNA-seq applications.
  • To highlight RNA-seq's advantages over array-based methods.

Main Methods:

  • RNA-sequencing (RNA-seq) for transcriptome profiling.
  • Comparative analysis with array-based methods.
  • Review of recent RNA-seq studies.

Main Results:

  • RNA-seq provides substantial contributions to understanding genome expression and regulation.
  • Recent studies reveal detailed information on transcriptional and post-transcriptional gene regulation.
  • Unprecedented resolution of transcript structures and processing is achieved.

Conclusions:

  • RNA-seq is a powerful technology for deep transcriptome sampling.
  • It offers unique insights into gene regulation and transcript complexity.
  • RNA-seq advances our understanding of biological systems at a global scale.