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The range of hemoglobin A(2) in hemoglobin E heterozygotes as determined by capillary electrophoresis

Daniel David Mais1, Ronald D Gulbranson, David F Keren

  • 1Department of Pathology, St Joseph Mercy Hospital, Ann Arbor, MI 48106, USA.

American Journal of Clinical Pathology
|October 30, 2009
PubMed

Capillary electrophoresis (CE) is capable of distinguishing hemoglobin E (HbE) from hemoglobin A(2) (HbA(2)), thus permitting quantification of HbA(2) in patients with HbE. In this study, routine samples submitted for evaluation of hemoglobinopathy that demonstrated HbE were studied by high-pressure liquid chromatography and CE. The data for 52 samples from adult HbE heterozygotes were compared with those for a control group consisting of 209 patients. The mean HbA(2) of patients with HbE trait was 3.4% (SD, 0.4%), which was significantly higher (P < .001) than the 2.6% (SD, 0.4%) for the control group. Seven samples from adults homozygous for HbE were also evaluated. The mean HbA(2) of HbE homozygotes was 4.4%, which was significantly greater (P < .001) than the HbA(2) values for the HbE heterozygotes. Data from these cases provide an estimate of the range of HbA(2) in patients with HbE when evaluated by CE.

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