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Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Published on: August 27, 2015

METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER.

¹Department of Biochemistry, Medical School, University of Otago, Dunedin, New Zealand.

The Journal of Cell Biology

|October 30, 2009

Summary

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Mouse liver cells maintain metabolic activity in specific sucrose solutions, but high concentrations inhibit respiration. Optimal conditions are crucial for preserving cell function and membrane integrity.

Area of Science:

Biochemistry
Cell Biology
Hepatology

Background:

Isolated liver cells are valuable for studying cellular metabolism.
Cellular metabolic activity is sensitive to the extracellular environment.

Purpose of the Study:

To investigate the impact of suspending medium composition on isolated mouse liver cell metabolic activity.
To determine optimal conditions for maintaining cell viability and function.

Main Methods:

Isolation of mouse liver cells via portal vein perfusion.
Incubation of cell suspensions in buffered media with varying sucrose concentrations (0.06-0.30 M).
Measurement of metabolic activities including respiration, substrate oxidation, and urea synthesis.

Main Results:

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Last Updated: Jun 19, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Published on: August 27, 2015

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

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Significant metabolic activity observed between 0.06-0.20 M sucrose; respiration ceased at 0.30 M.
Peak endogenous respiration occurred at ≤0.10 M sucrose, linked to acetoacetate formation.
Cells oxidized various metabolic substrates but not carbohydrates; urea synthesized from ammonium chloride.
No respiration in Krebs' phosphate-saline, attributed to membrane permeability loss, ion influx, and mitochondrial damage.
Leakage of soluble enzymes confirmed compromised cellular permeability barriers.

Conclusions:

The composition of the suspending medium critically influences isolated liver cell metabolic function.
Sucrose concentration impacts cell membrane integrity and mitochondrial respiration.
Krebs' phosphate-saline is unsuitable for maintaining isolated mouse liver cell viability due to membrane damage.