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Related Concept Videos

Liver Histology01:27

Liver Histology

The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
Hepatocytes perform a variety of essential functions. They secrete...

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Related Experiment Video

Updated: Jun 19, 2026

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
08:22

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Published on: December 16, 2021

FINE STRUCTURE OF CELLS ISOLATED FROM ADULT MOUSE LIVER.

M N Berry1, F O Simpson

  • 1Departments of Biochemistry and Medicine, Medical School, University of Otago, Dunedin, New Zealand.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

Isolated mouse liver cells lose membrane integrity in low osmolarity media. Mitochondrial structure changes significantly, impacting cell function and metabolic activity.

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Last Updated: Jun 19, 2026

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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
08:54

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Published on: February 12, 2018

Area of Science:

  • Cell Biology
  • Hepatology
  • Mitochondrial Research

Background:

  • Isolated liver cells are crucial for studying cellular functions and drug metabolism.
  • Maintaining cell viability and structural integrity after isolation is a significant challenge.
  • Understanding the effects of isolation and suspension media on cell structure is vital.

Purpose of the Study:

  • To investigate the fine structural changes in isolated mouse liver cells compared to intact liver.
  • To determine the impact of different osmolarity media on cell membrane and mitochondrial morphology.
  • To correlate observed structural changes with potential effects on metabolic activity.

Main Methods:

  • Mouse livers were perfused with a buffered medium containing 0.40 M sucrose.
  • Isolated liver cells were suspended in various media of differing osmolarities.
  • Cellular fine structure was examined using osmium tetroxide fixation and electron microscopy.

Main Results:

  • Perfusion separated cells with minimal membrane damage; however, isolation in 0.40 M sucrose caused plasma membrane breakdown.
  • Lower osmolarity media exacerbated plasma membrane disintegration, leading to loss of permeability barriers.
  • Mitochondria showed altered morphology, including cristae separation, swelling, and shape changes (spherical, crescent-shaped) in response to different sucrose concentrations.

Conclusions:

  • The osmolarity of the suspension medium critically affects the integrity of isolated liver cell plasma membranes.
  • Mitochondrial structural alterations in isolated liver cells are dependent on the external osmolarity.
  • These morphological changes likely influence the metabolic capacity and reliability of isolated liver cells in experimental settings.