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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
A METHOD FOR THE QUANTITATIVE ESTIMATION OF CYTOPLASMIC STRUCTURES
1Detroit Institute of Cancer Research, Detroit.
The Journal of Cell Biology
|October 30, 2009
Abstract:
A sampling procedure and calculations are described by which electron micrographs of cytoplasmic structures may be quantitatively analyzed. The relative areas occupied by formed bodies and by the "membrane space," the remainder of the cytoplasm, are evaluated. A method for making a measurement of the quantity of endoplasmic reticulum or other membrane profiles is described. The technic and results are illustrated with normal rat liver cells.

