Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A METHOD FOR THE QUANTITATIVE ESTIMATION OF CYTOPLASMIC STRUCTURES.

The Journal of cell biology·2009
Same author

Lack of Bcl11b tumor suppressor results in vulnerability to DNA replication stress and damages.

Oncogene·2007
Same author

Multi-step lymphomagenesis deduced from DNA changes in thymic lymphomas and atrophic thymuses at various times after gamma-irradiation.

Oncogene·2007
Same author

Enzymatic and genetic bases on assimilation, depolymerization, and transport of heteropolysaccharides in bacteria.

Journal of bioscience and bioengineering·2005
Same author

Rapid and sensitive neurotoxicity test based on the morphological changes of PC12 cells with simple computer-assisted image analysis.

Journal of bioscience and bioengineering·2005
Same author

p53 prevents maturation of T cell development to the immature CD4-CD8+ stage in Bcl11b-/- mice.

Biochemical and biophysical research communications·2005

Related Experiment Video

Updated: Jun 19, 2026

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

THE INDUCTION OF MELANIZATION IN GOLDFISH SCALES WITH ACTH, IN VITRO: Cellular and Subcellular Changes.

A V Loud1, Y Mishima

  • 1Detroit Institute of Cancer Research and the Department of Dermatology, Wayne State University, College of Medicine, and Detroit Receiving Hospital, Detroit, Michigan.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary

Adrenocorticotropic hormone (ACTH) can induce melanization in xanthic goldfish scales by promoting enzyme-substrate association. This study reveals similarities between yellow pigment cells and melanized cells, suggesting a potential pathway for pigment transformation.

More Related Videos

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
11:24

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

Published on: March 19, 2015

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
07:16

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish

Published on: March 1, 2022

Related Experiment Videos

Last Updated: Jun 19, 2026

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
11:24

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

Published on: March 19, 2015

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
07:16

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish

Published on: March 1, 2022

Area of Science:

  • Cell Biology
  • Pigment Cell Biology
  • Goldfish Physiology

Background:

  • Melanization is a critical process for pigment formation in many organisms.
  • Xanthic goldfish possess both yellow-pigmented cells (lipophores, lipocytes) and melanized cells (melanophores, melanocytes).
  • The role of ACTH in regulating pigment cell differentiation and function is of significant interest.

Purpose of the Study:

  • To investigate the in vitro induction of melanization in xanthic goldfish scales using ACTH.
  • To examine the structural similarities and differences between yellow pigment cells and melanized cells.
  • To elucidate the mechanism by which ACTH stimulates melanization at the cellular and subcellular levels.

Main Methods:

  • In vitro culture of xanthic goldfish scales.
  • Stimulation with ACTH.
  • Light and electron microscopy.
  • Ammoniated silver nitrate staining for premelanin and melanin.

Main Results:

  • Melanized cells (melanophores, melanocytes) and yellow pigment cells (lipophores, lipocytes) share structural similarities.
  • Lipocytes and lipophores contain cytoplasmic bodies that stain positively for premelanin.
  • ACTH stimulation in tissue culture suggests these bodies can undergo melanization.
  • Electron microscopy shows intermediate melanin granule formation and transitional melanolipophores.

Conclusions:

  • ACTH can induce melanization in goldfish scales.
  • Yellow pigment cells (lipocytes, lipophores) possess structures capable of becoming melanized.
  • It is postulated that ACTH facilitates the interaction of tyrosinase with unmelanized granules, initiating melanization.