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Related Experiment Video

Updated: Jun 19, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

RESPIRATION OF RAT PERITONEAL MAST CELLS.

N Chakravarty1, E Zeuthen

  • 1Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

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This study details microgasometry methods for mast cells using the Cartesian diver technique. Researchers measured mast cell respiration rates, finding glucose significantly increases oxygen consumption.

Area of Science:

  • Cellular biology
  • Biophysics

Background:

  • Mast cells play crucial roles in immune responses and inflammation.
  • Accurate measurement of cellular respiration is vital for understanding cell metabolism.

Purpose of the Study:

  • To describe a microgasometry method for quantifying mast cell respiration.
  • To determine the metabolic activity of mast cells with and without glucose.

Main Methods:

  • Utilized the Cartesian diver technique for microgasometry.
  • Quantified sample size by counting mast cells within the diver.
  • Measured respiration rates at 37°C.

Main Results:

  • Respiration rate without substrate: 0.29 x 10^-6 µL/cell/hour.
  • Respiration rate with glucose: 0.47 x 10^-6 µL/cell/hour.

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Last Updated: Jun 19, 2026

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  • Glucose significantly enhanced mast cell respiration.

    • Conclusions:

    • The Cartesian diver technique is effective for microgasometry of mast cells.
    • Mast cells exhibit increased metabolic activity in the presence of glucose.
    • This method provides a foundation for further studies on mast cell metabolism.