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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Published on: July 4, 2018
RESPIRATION OF RAT PERITONEAL MAST CELLS
1Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark.
The Journal of Cell Biology
|October 30, 2009
Abstract:
Methods for microgasometry of a few hundred mast cells are described. The Cartesian ampulla diver technique is used. The sample size is determined by counting the cells within the diver. The respiration rates at 37 degrees C, expressed in microliters per cell per hour, are 0.29 x 10(-6) without substrate and 0.47 x 10(-6) with glucose.
