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Related Experiment Video

Updated: Jun 19, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

GLYCOLYSIS IN RAT PERITONEAL MAST CELLS.

N Chakravarty1

  • 1Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

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Rat mast cells exhibit high glycolytic activity, significantly exceeding oxygen consumption. This indicates substantial glucose utilization, particularly under anaerobic conditions, highlighting their metabolic flexibility.

Area of Science:

  • Biochemistry
  • Cell Physiology

Background:

  • Mast cells play crucial roles in allergic and inflammatory responses.
  • Understanding mast cell metabolism is vital for developing targeted therapies.

Purpose of the Study:

  • To quantify the glycolytic activity of rat peritoneal mast cells.
  • To compare anaerobic and aerobic glycolysis rates.
  • To interpret these findings in the context of glucose utilization.

Main Methods:

  • Cartesian ampulla diver technique used for precise measurement.
  • Measurement of carbon dioxide (CO2) production in a bicarbonate medium.
  • Assessment of glycolysis with and without glucose, under both aerobic and anaerobic conditions.

Main Results:

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Last Updated: Jun 19, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

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Published on: July 4, 2018

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

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  • Anaerobic glycolysis rates were 1.70 x 10^-6 microl CO2/cell/hour (with glucose) and 1.43 x 10^-6 microl CO2/cell/hour (without glucose).
  • Aerobic glycolysis rate was 0.93 x 10^-6 microl CO2/cell/hour (with glucose).
  • CO2 production via anaerobic and non-respiratory aerobic glycolysis significantly exceeded previously reported oxygen uptake.

Conclusions:

  • Rat mast cells demonstrate high glycolytic rates.
  • These findings suggest a significant reliance on glucose metabolism for mast cell function.
  • The high CO2 production indicates substantial glucose utilization by these cells.