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Isolation of nuclei from yeast

M M Bhargava1, H O Halvorson

  • 1Laboratory of Molecular Biology and the Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706.

The Journal of Cell Biology
|October 30, 2009
PubMed
Summary

A new method efficiently isolates yeast nuclei, yielding a high DNA/protein ratio. These isolated nuclei exhibit key structural features and maintain RNA synthesis capabilities, proving useful for molecular studies.

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