Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intraepithelial anchoring fibril components.

L A Goldsmith1, P McCoon, A Partridge

  • 1Department of Dermatology, University of Rochester School of Medicine and Dentistry, NY 14642.

Archives of Dermatology
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vision-based manipulation of transparent plastic bags in industrial setups.

Frontiers in robotics and AI·2025
Same author

Early breast cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2023
Same author

The essential role of dermatology publications in enhancing professional diversity, equity, and inclusion.

Clinics in dermatology·2022
Same author

How to plan, do and report patient and public involvement in research.

Anaesthesia·2022
Same author

The essential role of dermatology publications in enhancing professional diversity, equity and inclusion.

The British journal of dermatology·2021
Same author

Dermatological education for the 21st century: prioritizing diversity.

The British journal of dermatology·2020
Same journal

Scoring With SCORTEN-Reply.

Archives of dermatology·2017
Same journal

Comparison of Early and Late Latent Syphilis- Colorado, 1991.

Archives of dermatology·2016
Same journal

Case of Rhinoscleroma.

Archives of dermatology·2016
Same journal

Cutaneous Fungal Infections.

Archives of dermatology·2016
Same journal

An Ulcerated Plaque in a Gay Man.

Archives of dermatology·2016
Same journal

Solitary Violaceous Nodule on the Face.

Archives of dermatology·2016
See all related articles

Researchers found intracellular anchoring fibril antigens in cultured human cells. These antigens may relate to recessive dystrophic epidermolysis bullosa, a skin disease characterized by anchoring fibril retention.

Area of Science:

  • Cell biology
  • Dermatology
  • Biochemistry

Background:

  • Anchoring fibrils are crucial components of the dermal-epidermal junction.
  • Defects in anchoring fibrils are associated with certain genetic skin disorders.

Purpose of the Study:

  • To investigate the presence and characteristics of intracellular anchoring fibril antigens in cultured human cells.
  • To explore the potential relationship between intracellular anchoring fibril antigens and recessive dystrophic epidermolysis bullosa.

Main Methods:

  • Utilized cultured human keratinocytes and ME-180 cervical carcinoma cells.
  • Employed anchoring fibril antibodies (AF1, AF2) for antigen detection.
  • Applied flow cytometry and acetone pretreatment to reveal intracellular antigens.

Related Experiment Videos

  • Assessed calcium sensitivity of the intracellular epitope.

    • Main Results:

    • Intracellular pools of antigens reacting with AF1 and AF2 antibodies were identified in both cell types.
    • In keratinocytes, these antigens formed a basement membrane-like structure.
    • Acetone pretreatment of ME-180 cells exposed large intracellular antigen pools.
    • The intracellular epitope demonstrated calcium sensitivity.

    Conclusions:

    • Cultured human cells contain intracellular anchoring fibril antigens.
    • The calcium-sensitive nature of these antigens was characterized.
    • Findings suggest a potential link between intracellular anchoring fibril antigens and recessive dystrophic epidermolysis bullosa, given its association with anchoring fibril retention.