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Updated: Jun 19, 2026

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS
S Kumar1, K I Loken, A J Kenyon
1Division of Veterinary Bacteriology and Public Health, University of Minnesota, St. Paul.
Abstract:
The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that rabbit immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin. Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities. Further studies dependent on the availability of purified alpha hemolysin are outlined.
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